General Cell Based Assays
Revvity Health Sciences Inc HTRF Human & Mouse PARP cleaved-Asp214 Detection Kit, 500 Assay Points
HTRF Human & Mouse PARP cleaved-Asp214 Detection Kit, 500 Assay Points
Cayman Chemical ERK/MAPK (Phospho-Thr202/Tyr204) Polyclonal Antibody, 1 ea
Antigen: phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr202 and phospho-Tyr204 of rat ERK/MAPK • Host: rabbit • Cross Reactivity: (+) human and rat ERK/MAPK • Application(s): WB • ERK/MAPK is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory. The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204.
Meso Scale Discovery R-PLEX Mouse M-CSF ASY
The R-PLEX Mouse M-CSF Assay from Meso Scale Diagnostics is a multiplex immunoassay kit for measuring mouse macrophage colony stimulating factor (M-CSF) in various biological fluids This ECL-based assay uses MULTI-ARRAY technology and includes a pre-coated 96-well plate calibrators controls diluent detection antibody read buffer and seals It has a sensitivity of 0 3 pg/mL and an upper limit of 10 000 pg/mL The assay offers high sensitivity low sample volume and quick results
Cayman Chemical GGT Substrate Diluent, 3.5 mL
GGT substrate diluent has been tested and formulated to work exclusively with Cayman’s γ-Glutamyl Transferase Activity Assay Kit (Item No. 702210).
Revvity Health Sciences Inc HTRF Human AQ STING Binding Kit, 500 Assay Points
HTRF Human AQ STING Binding Kit, 500 Assay Points
Montana Molecular Human Adenosine A1 Receptor (AA1R, ADORA1) Big Sky BacMam, 15mL
Human A1 Adenosine receptor packaged in Montana Molecular's Big Sky BacMam vector. Express the adenosine receptor in your cells of interest. Big Sky BacMam packaging provides efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in primary or iPSC-derived cells. BacMam expression is easily titrateable to suit your experimental needs.
Eurofins Discoverx PathHunter eXpress HCRTR2 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0427E2CP0M)
The PathHunter HCRTR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. orexin A) based activation of HCRTR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HCRTR2 and EA-tagged β-Arrestin-2. Activation of HCRTR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress ADRA2C CHO-K1 GPCR Assay, 10-Plate Kit. (95-0053E2CP2L)
The cAMP Hunter ADRA2C eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. epinephrine and norepinephrine) based activation of ADRA2C GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type ADRA2C, a naturally Gi/Go coupled GPCR. Upon activation of ADRA2C, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter TrkC Bioassay Kit, 10-Plate. (93-0464Y3-00078)
The PathHunter TrkC Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This cell line enables assessment of ligand (e.g. NT-3) based activation of TrkC receptors via detection of SH2 domain protein recruitment. The included cell line overexpresses ProLink-tagged TrkC target protein and EA-tagged SH2 domain protein. Upon activation of the receptor by its ligand or an agonist antibody, recruitment of SH2 domain protein to the phosphorylated receptor tail leads to production of EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Meso Scale Discovery U-PLEX Mouse IL-15 ASY 25 PL
The Meso Scale Discovery U-PLEX Mouse IL-15 Assay Kit is an electrochemiluminescent immunoassay for the quantitative measurement of mouse interleukin 15 (IL-15) in serum plasma and cell culture supernatants The assay uses MSD s proprietary SULFO-TAG labels and multi-array technology to provide sensitive and reproducible quantitation of mouse IL-15 with a lower limit of detection of 1 3 pg/mL and an upper limit of quantification of 10 000 pg/mL The kit includes a 96-well plate coated with capture antibody detection antibody conjugated to MSD SULFO-TAG calibrators and sample diluent The assay takes approximately 3 5 hours to complete after a 2 hour incubation
Eurofins Discoverx PathHunter eXpress GPR132 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0411E2ACP1M)
The PathHunter GPR132 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR132 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR132 and EA-tagged β-Arrestin-2. Activation of GPR132 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Sigma Aldrich Fine Chemicals Biosciences Direct DetectR Assay free
Quantity OptionsDDAC00010-GR 50 CardsDDAC00010-4P 200 CardsDDAC00010-8P 400 CardsThe Direct Detect Assay-free Cards are desgined for use in the the Direct Detect Spectrometer. The card contains four hydrophilic polytetrafluoroethylene (PTfE) membrane positions sized for easy sample application and surrounded by a hydrophobic ring to retain analyzed sample within the IR beam. A 2 muL sample volume is recommended in order to assure proper sample application drying and measurement.The Assay-free card can be inserted into the system and analyzed immediately after preparation. The Direct Detect spectrometer has a built in drying system that will automatically dry the sample to the point where it will give an accurate reading. The Direct Detect Assay-free card is intended for a one-time protein application however already prepared cards can be stored and reread later.
Eurofins Discoverx PathHunter eXpress SSTR5 U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0402E3CP0M)
The PathHunter SSTR5 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. somatostatin) based activation of SSTR5 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged SSTR5 and EA-tagged β-Arrestin-2. Activation of SSTR5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress PROKR1 Total GPCR Internalization Assay, 10pl Kit. (93-0764E3CP0L)
The PathHunter PROKR1 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of PROKR1 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged PROKR1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the PROKR1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR150 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0375E2ACP1L)
The PathHunter GPR150 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR150 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR150 and EA-tagged β-Arrestin-2. Activation of GPR150 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.